Transfer from callus plant culture to suspension culture (on shakers)


Service provides transferring callus culture cells from solid (agar) culture medium in liquid culture media and adapting cell growth under conditions of constant stirring in shaker. Callus cells as large and small cellular aggregates transferred to liquid culture medium in flasks Erlenmeyyera 250 ml and mounted on a shaker, where the clock mixing cells in a medium in thermal room at 24 ° in the dark. In such circumstances, large cell aggregates are crushed, and the rate of cell division increases. Passing several cycles of growth leads to homogeneity of culture-producer. It is possible to transfer to the two-stage cell culture growth medium in two forms: growth (for increasing cell mass) and productive (to stimulate synthesis of target substances). Implementation services can take, depending on the number of months to a year.