Development of Amyloid-Sensitive Fluorescent Dyes and Study of Specific Protein Aggregation

Many neurodegenerative diseases in humans result from protein misfolding and aggregation. Protein misfolding is thought to be the leading cause of Alzheimer's disease, Parkinson's disease, Huntington's disease, Creutzfeldt-Jakob disease, cystic fibrosis, Gaucher's disease and many other degenerative and neurodegenerative disorders which are collectively known as amyloid disorders.
Otava Institute provides the study and development of the amyloid-sensitive fluorescent dyes for protein aggregation. Futhermore, design of specific chemical inhibitors of protein aggregation. 

 

The service includes:

Development of fluorescent amyloid-sensitive dyes to detect different protein fibrillization products (i.e. mature fibrils, amorphous aggregates, oligomeric intermediates etc.) and monitor aggregation reaction with:

  • Required spectral characteristics (excitation/emission wavelength)
  • Increased sensitivity to your target protein

Development of fluorescent detection kits and analysis protocols based on the kits

Evaluation of compounds affecting the amyloid aggregation of your target protein:

  • Screening of compounds of different chemical classes
  • Modeling of aggregated protein-ligand complexes

Chemical optimization of the lead compounds