Design and development of fluorescent dyes with requested characteristics for biomolecules sensing
Otava institute offers a broad range of high quality fluorescent dyes for various bioanalytical applications. The approaches for both qualitative and quantitative detection of the biomolecules are based on:
- An ability of the weakly emissive in unbound state dye to convert in a strongly emissive state when bound to specific biomolecule. Among the targets are such fluorescent light-up probes:
- nucleic acids (ssDNA, dsDNA, RNA);
- protein aggregates (including protein oligomers and amyloid fibrils);
- proteins and peptides.
- Labeling of biomolecules by high-emissive fluorescent dye upon their non-covalent binding.
- Labeling of biomolecules by high-emission fluorescent dye by covalent stitching.
We propose variety of dyes, which are the derivatives of the cyanine, styrylcyanine, squaraine and … families. This unique vast collection of dyes allows choosing the dye for specific bioapplication with desired characteristics such as Stock shifts, excitation/emission wavelength, charge, hydrophilic/hydrophobic properties, etc. Some of dyes are optimized for the red range of the spectrum so that they can be used for the biological samples with auto-fluorescence. Large synthetic experiences of our chemists allow performing modifications of “lead” dyes to increase their affinity/selectivity to the target biomolecules.
*Our scientists with a thorough experience in the field of fluorescent probes for biomedical applications will provide you with consultative and practical assistance in development of novel fluorescent probes for application in diagnostic systems, elaboration of new methods for oligonucleotides and proteins labeling by polymethine dyes.